ROSOLIC ACID (0.1 gm/vl)

Rehydrate the contents of one vial aseptically with 10 ml of 0.2 N sterile Sodium hydroxide. Mix well and aseptically add it to 990 ml of TM 178 – M-FC AGAR BASE / TMV 178 – M-FC AGAR BASE (VEG.) / TM 180 – M-FC BROTH BASE before boiling. Add 10 ml of 1% Rosolic Acid to sterile, molten, cooled (45-50?C) TM 179 – M-FC AGAR BASE MODIFIED along with 50 mg Carbenicillin. Mix well and dispense as desired.

For isolation of coliform bacteria.

coliforms

Water

5 vl