ANTIBIOTIC ASSAY MEDIUM NO. 13 (as per USP)

This medium is formulated in accordance to USP and CFR and is numerically identical with the name assigned by Groove and Rundall. Schmidt & Moyer has reported the use of antibiotic assay medium for liquid formulation in performance of antibiotic assay. This medium is widely used in turbidometric assay of antifungals like candicidin using test organism like Saccharomyces cerevisiae. The is medium is also termed as Sabouraud Liquid Broth Modified or Fluid Sabouraud Medium. This medium facilitates enhanced growth of test organism Saccharomyces cerevisiae employed in assay of candicidin, a polyene antibiotic with antifungal activity. Assay is performed by enumerating the blastospores or by analysing the turbidity of the medium. Turbidimetric antibiotic assay is based on the change or inhibition of growth of a test microorganism in a liquid medium containing a uniform concentration of an antibiotic. After incubation of the test organism in the working dilutions of the antibiotics, the amount of growth is determined by measuring the light transmittance using spectrophotometer. The concentration of antibiotic is determined by comparing amounts of growth obtained with that given by the reference standard solutions. Use of this method is appropriate only when test samples are clear.

for turbidimetric microbiological assay of Candicidin using Saccharomyces cerevisiae and to study the e?ectiveness of antibiotics on yeasts and molds

Saccharomyces cerevisiae

Pharmaceutical

USP

500 gm