ETHYL VIOLET AZIDE DEXTROSE AGAR

Ethyl Violet Azide Broth is based on the formulation of Litsky et al and is a modification of medium developed by Litsky et al with reduced amount of dextrose and increased dye concentration, making the medium highly specific for Enterococci. The presence of Enterococci acts as a valuable index of faecal or sewage pollution in water. Ethyl Violet Azide Dextrose Agar is a modification of Ethyl Violet Azide Broth where 1.5% agar is added as a solidifying agent. It is used for detection and confirmation of Streptococci. It is based on original formulation of Litsky et al. Ethyl Violet Azide Dextrose Agar medium has 0.5% dextrose and was found equally productive as the medium described originally containing 1.5% dextrose. It was found that the medium with the lesser amount of carbohydrate was less adversely affected by heat during sterilization. Litsky et al studied a variety of dyes and selective agents for Streptococci and developed a confirmatory medium using ethyl violet and sodium azide as selective agents. Combination of 0.0083gm% of ethyl violet dye and 0.04gm% of azide provided the best selective action favouring growth of Streptococci.

for detecting & con?rming Streptococci and faecal pollution indication in water

Enterococcus faecalis

Water

500 gm