TINSDALE AGAR BASE

The Corynebacteria are gram-positive, non-sporulating, non-motile rods. They are often club-shaped and frequently banded or beaded with irregularly stained granules. These bacteria are generally aerobic or facultative, but microaerophilic species do occur. Corynebacterium diphtheriae produces a powerful exotoxin that causes diphtheria in humans. In nature, C. diphtheriae occurs in nasopharyngeal area of infected persons or healthy carriers. The three biotypes of C. diphtheriae are mitis, intermedius and gravis. The signs and symptoms of diphtheria are sore throat, malaise, headache and nausea. Tinsdale Agar Base Medium was developed by Tinsdale for the selective isolation and differentiation of C. diphtheriae from diphtheroids. This medium was modified by Billings, which improved the recovery and differential qualities of C. diphtheriae. The present medium is according to the modified Billings Medium. Moore and Parsons confirmed the halo formation as a characteristic property of C. diphtheriae with the exception of C. ulcerans, which forms colony with similar features as C. diphtheriae.

for selective isolation and di?erentiation of Corynebacterium diphtheriae

Corynebacterium diphtheriae | Streptococcus pyogenes

Clinical Diagnostics

500 gm