ANAEROBIC AGAR (BREWER)

Brewer devised this medium for use with Brewer anaerobic cover to permit surface growth of anaerobes and microaerophiles on agar without the use of anaerobic jar. This medium is suitable for isolation of facultative and obligate anaerobes and for the study of colonial morphology as colonies can be readily seen on the light coloured agar and are easily accessible. Dispense 50-60 ml medium per 95 x 20 mm plate. For best results, use porous tops for the plates during solidification to obtain a dry surface. Inoculation can be done by streaking or smearing. After inoculation of the medium, cover with Brewer Anaerobic Petri dish cover. The sealing ring inside the cover should make a perfect contact with the medium and must not be broken before the end of the incubation period. When standard plates are used, dispense 0.1 to 1.0 ml of inoculum into plates and mix 20-25 ml of sterile medium.

for isolation and sensitivity testing of anaerobic and microaerophilic organisms

Clostridium botulinum | Clostridium perfringens | Clostridium sporogenes

Cosmetics | Veterinary

500 gm