BLOOD AGAR BASE No. 2

A fastidious organism is one with complete nutritional requirements, needing additional cellular building-block molecules in order to survive. Blood Agar Base No. 2 is a highly nutritive medium. Microorganisms producing haemolysin give visible haemolytic zones on this medium. It also serves as a differential medium for Brucella and Campylobacter species by adding different antibiotic supplements for the respective bacteria. Brucella cultures are highly infective and must be handled with care. Incubate preferably in 5-10% carbon dioxide atmosphere. Comparative studies of horse, rabbit and sheep blood showed that sheep blood gave the clearest and most reliable colony and haemolysis characteristics at both 24 and 48 hours of incubation. It can be used to prepare Chocolate Agar for the isolation of Haemophilus and Neisseria species. It can also be used for primary isolation of Haemophilus species, where horse blood is used for enrichment. Better results are obtained by spreading half of the horse blood agar plate with 2 drops of 10% saponin.

for isolation, cultivation and detection of haemolytic activity of Streptococci, Pneumococci and other fastidious microorganisms

Neisseria meningitidis | Staphylococcus aureus subsp. aureus | Streptococcus pneumoniae | Streptococcus pyogenes

Clinical Diagnostics | Industrial & Lab Purposes | Veterinary

100 gm | 500 gm