Description
Eugonic Agar was developed by Pelczar and Vera for cultivation of fastidious organisms like Brucella. These media can also be used to grow Mycobacteria and various pathogenic fungi including Nocardia, Histoplasma and Blastomyces, when enriched with blood. Niven used this media for detection of spoilage of meats. Eugonic Agar was developed to obtain eugonic (luxuriant) growth of fastidious microorganisms like Brucella that are otherwise difficult to cultivate. The unnourished medium supports rapid growth of lactobacilli associated with cured meat products, dairy products and other foods. APHA recommends Eugonic agar, which is also used in germinating anaerobic spores pasteurized at 104?C. Eugonic Agar is quite similar to Tryptone Soya Agar but more bacterial propagation is expected on Eugonic Agar. Organisms like Bordetella and Neisseria form minute colonies on Tryptone Soya Agar. They may become large on Eugonic agar because large amount of sulfur and carbon sources have been added in addition to the Tryptone Soya Agar formula. Therefore, this medium is recommended for the direct isolation of Bordetella pertussis and Neisseria meningitides from the test materials such as throat mucus, blood, cerebrospinal fluid, pleural fluid and other specimens. For the isolation of Bacillus pumilus, Eugonic Agar can be supplemented with 0.1% starch, prior to sterilization.
Principle
for cultivation of fastidious microorganisms like Haemophilus, Neisseria, Pasteurella, Brucella and Lactobacillus species
