HIGH PLATE COUNT AGAR

The heterotrophic plate count (HPC), formerly known as the standard plate count is a procedure for estimating the numbers of live heterotrophic bacteria in water and measuring the changes during water treatment and distribution or in swimming pools. Different methods namely pour plate method, spread plate method and membrane filter method can be followed to obtain heterotrophic plate count. High Plate Count Agar is recommended by APHA for determining heterotrophic plate count. This low nutrient medium is likely to produce higher colony counts than high nutrient media.

for obtaining higher colony counts by spread plate or pour plate or membrane ?lter technique

Bacillus subtilis subsp. spizizenii | Enterococcus faecalis | Escherichia coli | Lactobacillus casei | Staphylococcus aureus subsp. aureus | Streptococcus pyogenes

Water

500 gm