R-3A AGAR

R-3A Agar is slightly more nutritious than R-2A Agar and is used for subculturing the isolates obtained on the less nutritive R-2A Agar. R-2A Agar is recommended by APHA for estimating the heterotrophic plate count by the pour plate, spread plate or membrane filter procedure. R-2A Agar is formulated as per Reasoner and Geldreich. Stressed or injured organisms during water treatment are unable to grow on high nutrient media, since the faster growing organisms outgrow the former. Therefore, the use of a low nutrient medium like R-2A Agar incubated for longer incubation periods allows these stressed organisms to grow well.

for subculturing of microorganisms recovered on less nutritive R-2A Agar from potable water

Candida albicans | Enterococcus faecalis | Escherichia coli | Salmonella Enteritidis | Salmonella typhi

Water

500 gm