SLANETZ AND BARTLEY MEDIUM

Slanetz and Bartley Medium was originally devised by Slanetz and Bartley for the detection and enumeration of Enterococci by membrane filtration technique. It can be also used as a direct plating medium. The medium is highly selective for Enterococci. The Department of Health has recommended this medium to be used for enumeration of Enterococci in water supplies. Water is filtered through a membrane filter which is then placed on the surface of the Slanetz and Bartley Medium plates and incubated at 35?C for 4 hours and then at 44-45?C for 44-48 hours. Red or maroon colonies are counted as Enterococci. The preliminary incubation at 35?C helps for the recovery of stressed organisms. Not all the species reduce TTC, hence pale colonies also should be considered.

for detection and enumeration of faecal Streptococci by membrane ?ltration technique

Enterococcus faecalis

Water

500 gm