YEAST MORPHOLOGY AGAR *

Yeasts are ubiquitous in our environment, being found on fruits, vegetables and plant materials. Yeasts are unicellular, eukaryotic, budding cells that are generally round to oval or, less often, elongated or irregular in shape. Colonies of yeasts have a smooth to wrinkled, creamy appearance. Yeast Morphology Agar is formulated as described by Wickerham. The medium plates are inoculated by the Dolmans technique. Using a light inoculum from an actively growing culture, smear a single line at one end of the plate and in two separate points at the opposite end. Place two sterile slides, one on the central section of the smear and one on one of the two-punctiform inoculate. After an incubation of 72-96 hours, take off the growth of the point inoculations and the smear without the slide and observe the morphology of the vegetative cells under a microscope. Also observe the zone underlying the slides for the formation of mycelium or pseudo mycelium under the microscope. Observe the colonial morphology.

for classi?cation of yeasts on the basis of their colonial characteristics and cell morphology

Candida albicans | Kloeckera apiculata | Saccharomyces uvarum

Clinical Diagnostics | Food & Beverages | Industrial & Lab Purposes

100 gm